Expression of toll-like receptor 2 on gamma delta T cells bearing invariant V gamma 6/V delta 1 induced by Escherichia coli infection in mice

J Immunol. 2000 Jul 15;165(2):931-40. doi: 10.4049/jimmunol.165.2.931.

Abstract

We recently reported that the number of gamma delta T cells was increased after infection with Escherichia coli in C3H/HeN mice. We here showed that an i.p. injection with native lipid A derived from E. coli induced an increase of gamma delta T cells in the peritoneal cavity of LPS-responsive C3H/HeN mice and, albeit to a lesser degree, also in LPS-hyporesponsive C3H/HeJ mice. The purified gamma delta T cells from C3H/HeN and C3H/HeJ mice expressed a canonical TCR repertoire encoded by V gamma 6-J gamma 1/V delta 1-D delta 2-J delta 2 gene segments and proliferated in response to the native lipid A derived from E. coli in a TCR-independent manner. The lipid A-reactive gamma delta T cells bearing canonical V gamma 6/V delta 1 expressed Toll-like receptor (TLR) 2 mRNA, while TLR4 mRNA was undetectable. Treatment with a TLR2 anti-sense oligonucleotide resulted in hyporesponsiveness of the gamma delta T cells to the native lipid A. TLR2-deficient mice showed an impaired increase of the gamma delta T cells following injection of native lipid A. These results suggest that TLR2 is involved in the activation of canonical V gamma 6/V delta 1 T cells by native E. coli lipid A.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Ascitic Fluid / immunology
  • Ascitic Fluid / metabolism
  • Cell Line
  • Cytokines / biosynthesis
  • Drosophila Proteins*
  • Escherichia coli Infections / immunology*
  • Escherichia coli Infections / metabolism
  • Female
  • Gene Expression Regulation / immunology
  • Genes, T-Cell Receptor delta
  • Genes, T-Cell Receptor gamma
  • Injections, Intraperitoneal
  • Lipid A / administration & dosage
  • Lipid A / physiology
  • Lipopolysaccharide Receptors / biosynthesis
  • Lipopolysaccharide Receptors / metabolism
  • Lipopolysaccharides / metabolism
  • Lipopolysaccharides / pharmacology
  • Lymphocyte Activation
  • Membrane Glycoproteins / biosynthesis*
  • Membrane Glycoproteins / genetics
  • Membrane Glycoproteins / physiology
  • Mice
  • Mice, Inbred C3H
  • Mice, Inbred C57BL
  • Mice, Knockout
  • Peritoneal Cavity / cytology
  • RNA, Messenger / biosynthesis
  • Receptors, Antigen, T-Cell, gamma-delta / biosynthesis*
  • Receptors, Antigen, T-Cell, gamma-delta / genetics
  • Receptors, Cell Surface / biosynthesis*
  • Receptors, Cell Surface / genetics
  • Receptors, Cell Surface / physiology
  • T-Lymphocyte Subsets / metabolism*
  • Toll-Like Receptor 2
  • Toll-Like Receptor 4
  • Toll-Like Receptors

Substances

  • Cytokines
  • Drosophila Proteins
  • Lipid A
  • Lipopolysaccharide Receptors
  • Lipopolysaccharides
  • Membrane Glycoproteins
  • RNA, Messenger
  • Receptors, Antigen, T-Cell, gamma-delta
  • Receptors, Cell Surface
  • Toll-Like Receptor 2
  • Toll-Like Receptor 4
  • Toll-Like Receptors