Abstract
To investigate the molecular mechanisms of muscle atrophy under microgravity, the paraspinal muscles of rats after 14 days spaceflight and those of ground-based controls were examined. In the microgravitational environment, expressions of 42 genes changed, and the expressions of heat shock protein 70 and t complex polypeptide 1 increased. In Northern blotting, myocyte-specific enhancer binding factor 2C (MEF2C) and MEF2C-related genes including aldolase A and muscle ankyrin decreased. After 9 days ground recovery, expression of MEF2C increased and it was located mainly on the satellite cells in the muscle regeneration state. MEF2C could be a key transcriptional factor for skeletal muscle atrophy and regeneration under microgravity.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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DNA / genetics
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DNA / metabolism
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Down-Regulation*
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Gene Expression Profiling
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MEF2 Transcription Factors
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Male
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Microscopy, Fluorescence
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Muscle Fibers, Slow-Twitch / metabolism
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Muscle Fibers, Slow-Twitch / pathology
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Muscle, Skeletal / metabolism*
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Muscle, Skeletal / pathology*
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Muscle, Skeletal / physiology
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Muscular Atrophy / genetics
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Muscular Atrophy / metabolism*
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Myogenic Regulatory Factors / genetics
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Myogenic Regulatory Factors / metabolism*
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Myosin Heavy Chains / genetics
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Myosin Heavy Chains / metabolism
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RNA, Messenger / genetics
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RNA, Messenger / metabolism
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Rats
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Rats, Sprague-Dawley
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Regeneration
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Space Flight
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Time Factors
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Weightlessness / adverse effects*
Substances
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MEF2 Transcription Factors
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Myogenic Regulatory Factors
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RNA, Messenger
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DNA
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Myosin Heavy Chains