[Effects of protein kinase C on non-heart organ protection of preconditioning]

Zhonghua Yi Xue Za Zhi. 1998 Jun;78(6):426-9.
[Article in Chinese]

Abstract

Objective: To investigate the effects of protein kinase C (PKC) on ischemic (hypoxic) preconditioning (PC) in the non-heart organs and tissue.

Methods: In the models of ischemia/reperfusion of in situ perfused rat small intestine, limb, and in the model of hypoxia/reoxygenation of cultured rat aortic vascular smooth muscle cells (VSMC), the effects of PKC on ischemic (hypoxic) PC were observed.

Results: PKC inhibitor H7 and polymyxin B completely abolished the protective effects of ischemic PC on small intestine and limb subjected to ischemia/reperfusion injury. Hypoxic PC activated PKC in VSMC and potentiated phosphorylation mediated by PKC.

Conclusion: The activation of PKC is also the important chain of PC on non-heart organs, and its mechanism might involve protein phosphorylation.

Publication types

  • English Abstract

MeSH terms

  • Animals
  • Cells, Cultured
  • Extremities / blood supply
  • Intestine, Small / blood supply*
  • Ischemic Preconditioning*
  • Male
  • Muscle, Smooth, Vascular / cytology
  • Protein Kinase C / pharmacology*
  • Rats
  • Rats, Wistar
  • Reperfusion Injury / prevention & control*

Substances

  • Protein Kinase C