Rapid molecular typing of Listeria monocytogenes by pulsed-field gel electrophoresis

K Katsuda; M Iguchi; T Tuboi; K Nishimori; K Tanaka; I Uchida; M Eguchi

doi:10.1053/rvsc.2000.0394

Rapid molecular typing of Listeria monocytogenes by pulsed-field gel electrophoresis

Res Vet Sci. 2000 Aug;69(1):99-100. doi: 10.1053/rvsc.2000.0394.

Authors

K Katsuda¹, M Iguchi, T Tuboi, K Nishimori, K Tanaka, I Uchida, M Eguchi

Affiliation

¹ Department of Systematic Diagnosis, National Institute of Animal Health, Tsukuba, Ibaraki, Japan. [email protected]

PMID: 10924402
DOI: 10.1053/rvsc.2000.0394

Abstract

Pulsed-field gel electrophoresis (PFGE) is a highly discriminating tool for molecular typing, but the conventional PFGE protocol is time consuming. This paper describes a rapid method of PFGE for Listeria monocytogenes that yields results within 2 days.

MeSH terms

DNA, Bacterial / chemistry
DNA, Bacterial / isolation & purification
Deoxyribonucleases, Type II Site-Specific / chemistry
Electrophoresis, Gel, Pulsed-Field / methods*
Food Microbiology*
Listeria monocytogenes / classification*
Listeria monocytogenes / genetics

Substances

DNA, Bacterial
CCCGGG-specific type II deoxyribonucleases
Deoxyribonucleases, Type II Site-Specific
GGGCCC-specific type II deoxyribonucleases