We previously determined a contiguous 1,025,415-nucleotide sequence of the entire human immunoglobulin lambda gene locus, in which a total of 36 potentially functional Vlambda genes and 33 pseudogenes were localized. We also identified many more incomplete Vlambda genes to be characterized further. Some of these possessed only a slight sequence homology with the known Vlambda genes, and others possessed a high homology but had severely truncated coding regions. Here, we made extensive characterization of 50 new Vlambda pseudogenes, totaling 119 gene segments in the Vlambda gene locus. Of these 119 Vlambda genes, 118 were localized within the five Vlambda gene-rich clusters that we previously defined. Two of these novel Vlambda pseudogenes possessed the opposite transcriptional polarity to all the other Vlambda genes. The present comprehensive analysis of 119 Vlambda genes validated our previous classification of Vlambda genes and provided a basis for a possible mechanism by which a large number of Vlambda pseudogenes were propagated and maintained as a particular locus during evolution.