The cultivation of encapsulated S. cerevisiate and C. utilis in NaCS-PDMDAAC Polyelectrolyte Complexes was studied. The results showed that these two kinds of encapsulated microorganisms showed the same growing properties as in their free cell cultures, and thus NaCS-PDMDAAC microcapsules were suitable for the encapsulation of biological substances. The encapsulated S. cerevisiate cells were fermented sequentially for 16 batches. The highest cell density in the capsules reached 2.64 x 10(10) cells/mL and ethanol concentration reached 47.0 g/L. The encapsulated C. utilis produced the same amount of GSH as the free cell culture.