Rapid degradation of ferulic acid via 4-vinylguaiacol and vanillin by a newly isolated strain of bacillus coagulans

J Biotechnol. 2000 Jul 14;80(3):195-202. doi: 10.1016/s0168-1656(00)00248-0.

Abstract

A new strain Bacillus coagulans BK07 was isolated from decomposed wood-bark, based on its ability to grow on ferulic acid as a sole carbon source. This strain rapidly decarboxylated ferulic acid to 4-vinylguaiacol, which was immediately converted to vanillin and then oxidized to vanillic acid. Vanillic acid was further demethylated to protocatechuic acid. Above 95% substrate degradation was obtained within 7 h of growth on ferulic acid medium, which is the shortest period of time reported to date. The major degradation products, was isolated and identified by thin-layer chromatography, high performance liquid chromatography and 1H-nuclear magnetic resonance spectroscopy were 4-vinylguaiacol, vanillin, vanillic acid and protocatechuic acid.

MeSH terms

  • Bacillus / classification
  • Bacillus / metabolism*
  • Benzaldehydes / metabolism*
  • Biodegradation, Environmental*
  • Biotechnology / methods
  • Chromatography, High Pressure Liquid
  • Coumaric Acids / chemistry
  • Coumaric Acids / pharmacokinetics*
  • Guaiacol / analogs & derivatives*
  • Guaiacol / metabolism*
  • Magnetic Resonance Spectroscopy
  • Trees / microbiology
  • Wood

Substances

  • Benzaldehydes
  • Coumaric Acids
  • Guaiacol
  • 4-vinylguaiacol
  • ferulic acid
  • vanillin