Detection and investigation of Campylobacter jejuni by polymerase chain reaction-restriction fragment length polymorphism analysis

A Kiuchi; M Hara; H S Pham; K Takikawa; R Itoh; K Tabuchi

Detection and investigation of Campylobacter jejuni by polymerase chain reaction-restriction fragment length polymorphism analysis

Microbios. 2000;102(403):159-64.

Authors

A Kiuchi¹, M Hara, H S Pham, K Takikawa, R Itoh, K Tabuchi

Affiliation

¹ Department of Veterinary Microbiology, School of Veterinary Medicine, Azabu University, Sagamihara Kanagawa, Japan.

PMID: 10955830

Abstract

A molecular typing approach for Campylobacter jejuni with restriction fragment length polymorphism (RFLP) analysis of the flagellin gene flaA in C. jejuni, was generated and studied. Using polymerase chain reaction (PCR)-RFLP with the restriction endonuclease Mbo I, it was demonstrated that C. jejuni could be divided into four types. Genotypic analysis of C. jejuni by PCR-RFLP is a valuable technique for epidemiological typing.

Publication types

Evaluation Study

MeSH terms

Animals
Bacterial Typing Techniques
Base Sequence
Campylobacter Infections / microbiology
Campylobacter Infections / veterinary*
Campylobacter jejuni / classification
Campylobacter jejuni / genetics
Campylobacter jejuni / isolation & purification*
Chickens
DNA, Bacterial / analysis
Deoxyribonucleases, Type II Site-Specific / metabolism
Flagellin / genetics
Genotype
Molecular Sequence Data
Polymerase Chain Reaction*
Polymorphism, Restriction Fragment Length*
Poultry Diseases / microbiology*

Substances

DNA, Bacterial
Flagellin
Deoxyribonucleases, Type II Site-Specific
GATC-specific type II deoxyribonucleases