Evidence of the existence of a new circulating recombinant form of HIV type 1 subtype A/J in Cameroon. The European Network on the Study of In Utero Transmission of HIV-1

AIDS Res Hum Retroviruses. 2000 Sep 1;16(13):1313-8. doi: 10.1089/08892220050117078.

Abstract

Several genetic subtypes and circulating recombinant forms (CRFs) of HIV-1 have been identified. The greatest degree of genetic diversity is displayed by variants from Central and West Africa. HIV-1 env C2-V5 and protease sequences were obtained from 15 HIV-1-infected pregnant women, who were selected from a larger cohort study in Yaoundé, Cameroon. Fourteen of 15 virus variants were shown to be recombinant, whereas a single variant appeared to be nonrecombinant subtype A. Five viruses were subtype A/J recombinants, with env genes derived from subtype A and protease genes derived from subtype J. Seven viruses clustered with reference sequences for CRF02 AG(IbNG) in both the env and protease gene fragments, and were thus subtype A/G recombinants. Two variants displayed even more complex recombination patterns. Phylogenetic analyses indicated that the five subtype A/J recombinants might be the first representatives of a previously unrecognized CRF.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cameroon / epidemiology
  • DNA, Viral / blood
  • Female
  • Gene Products, env / genetics
  • Genetic Variation
  • HIV Infections / epidemiology
  • HIV Infections / virology*
  • HIV Protease / genetics
  • HIV-1 / classification*
  • HIV-1 / genetics*
  • Humans
  • Molecular Sequence Data
  • Phylogeny
  • Pregnancy
  • Pregnancy Complications, Infectious / virology*
  • Recombination, Genetic / genetics*
  • Sequence Analysis, DNA

Substances

  • DNA, Viral
  • Gene Products, env
  • HIV Protease

Associated data

  • GENBANK/AF268325
  • GENBANK/AF268326
  • GENBANK/AF268327
  • GENBANK/AF268328
  • GENBANK/AF268329
  • GENBANK/AF268330
  • GENBANK/AF268331
  • GENBANK/AF268332
  • GENBANK/AF268333
  • GENBANK/AF268334
  • GENBANK/AF268335
  • GENBANK/AF268336
  • GENBANK/AF268337
  • GENBANK/AF268338
  • GENBANK/AF268339
  • GENBANK/AF268340
  • GENBANK/AF268341
  • GENBANK/AF268342
  • GENBANK/AF268343
  • GENBANK/AF268344
  • GENBANK/AF268345
  • GENBANK/AF268346
  • GENBANK/AF268347
  • GENBANK/AF268348
  • GENBANK/AF268349
  • GENBANK/AF268350
  • GENBANK/AF268351
  • GENBANK/AF268352
  • GENBANK/AF268353
  • GENBANK/AF268354