An improved rapid procedure for fluorescence in situ hybridization that is applicable to intraoperative cancer cytodiagnosis

K Koide; C Sakakura; A Hagiwara; T Yamaguchi; H Yamagishi; T Abe; J Inazawa

doi:10.1016/s0304-3835(00)00516-4

An improved rapid procedure for fluorescence in situ hybridization that is applicable to intraoperative cancer cytodiagnosis

Cancer Lett. 2000 Oct 1;158(2):165-9. doi: 10.1016/s0304-3835(00)00516-4.

Authors

K Koide¹, C Sakakura, A Hagiwara, T Yamaguchi, H Yamagishi, T Abe, J Inazawa

Affiliation

¹ Department of Digestive Surgery, Kyoto Prefectural University of Medicine, 465 Kajii-cho, Hirokohji, Kawaramachi-tori, Kamigyo-ku, Kyoto, Japan. [email protected]

PMID: 10960766
DOI: 10.1016/s0304-3835(00)00516-4

Abstract

Fluorescence in situ hybridization (FISH) is among the most simple and useful methods for detecting numerical and structural aberration of chromosomes but it requires 12-24 h to complete. We devised a rapid FISH method that can be performed within 2 h. Here we describe the technique, which we have found to be extreme simple and as sensitive and specific as standard FISH, making it highly suitable for clinical use.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Adult
Aged
Aged, 80 and over
Allelic Imbalance
Chromosome Aberrations
Chromosomes, Human, Pair 1 / genetics
Chromosomes, Human, Pair 11 / genetics
Chromosomes, Human, Pair 17 / genetics
Chromosomes, Human, Pair 18 / genetics
Colorectal Neoplasms / genetics
Colorectal Neoplasms / pathology
Cytodiagnosis*
Diagnosis, Differential
Esophageal Neoplasms / genetics
Esophageal Neoplasms / pathology
Female
Humans
In Situ Hybridization, Fluorescence / methods*
Male
Middle Aged
Neoplasms / genetics
Neoplasms / pathology*
Sensitivity and Specificity
Stomach Neoplasms / genetics
Stomach Neoplasms / pathology
Time Factors
U937 Cells
Y Chromosome / genetics