Abstract
Protein phosphatase inhibitor-1 (inhibitor-1 or I-1) is involved in signal transduction and is an endogenous inhibitor of protein phosphatase-1. The mouse I-1 protein sequence has been deduced from cDNA and is strongly homologous to the published rat sequence. A mouse genomic library was screened, and the I-1 gene was characterized and localized by fluorescent in situ hybridization (FISH) to chromosome 15F. Protein expression in a range of embryonic and adult tissue was analysed using confocal microscopy. Inhibitor-1 is expressed by: the coelomic epithelium; the epithelial bounding layer of cells of the kidney, lung, liver, heart, intestine and gonad; and the surface ectoderm. The blast cells of the kidney do not express I-1. We conclude that I-1 is a marker for mesothelium.
Publication types
-
Research Support, Non-U.S. Gov't
MeSH terms
-
Amino Acid Sequence
-
Animals
-
Carrier Proteins*
-
Enzyme Inhibitors / metabolism
-
Epithelium / embryology*
-
Epithelium / metabolism*
-
Gene Expression Regulation, Developmental
-
Genetic Markers
-
Immunohistochemistry
-
In Situ Hybridization, Fluorescence
-
Intracellular Signaling Peptides and Proteins*
-
Kidney / embryology
-
Kidney / metabolism
-
Male
-
Mice
-
Molecular Sequence Data
-
Phosphoprotein Phosphatases / antagonists & inhibitors*
-
Protein Phosphatase 1
-
RNA, Messenger / genetics
-
RNA, Messenger / metabolism
-
RNA-Binding Proteins / genetics*
-
RNA-Binding Proteins / metabolism
-
Rats
-
Sequence Homology, Amino Acid
-
Tissue Distribution
Substances
-
Carrier Proteins
-
Enzyme Inhibitors
-
Genetic Markers
-
Intracellular Signaling Peptides and Proteins
-
RNA, Messenger
-
RNA-Binding Proteins
-
protein phosphatase inhibitor-1
-
Phosphoprotein Phosphatases
-
Protein Phosphatase 1