Serum-induced hypha formation in the dimorphic yeast Yarrowia lipolytica

FEMS Microbiol Lett. 2000 Sep 1;190(1):9-12. doi: 10.1111/j.1574-6968.2000.tb09254.x.

Abstract

The dimorphic yeast Yarrowia lipolytica forms true hyphae in a medium containing N-acetylglucosamine. We made a new finding that serum is a very effective inducer of hypha formation of Y. lipolytica: serum induced its hyphal growth very quickly compared to N-acetylglucosamine (4 h vs. 10 h). Osmotic and oxidative stresses (0.2 M NaCl and 20 mM H2O2) inhibited the hypha formation induced by N-acetylglucosamine, but did not suppress the hypha formation triggered by serum. Serum-specific morphological mutants, which formed hyphae in the N-acetylglucosamine medium but not in serum medium, could be isolated. These results suggest that the signal triggered by serum may be transduced through a different pathway, at least in part, from that used for the N-acetylglucosamine signal in Y. lipolytica.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Acetylglucosamine / pharmacology
  • Blood
  • Culture Media / chemistry
  • Osmotic Pressure
  • Oxidative Stress
  • Saccharomycetales / drug effects
  • Saccharomycetales / genetics
  • Saccharomycetales / growth & development*
  • Yeasts / drug effects
  • Yeasts / genetics
  • Yeasts / growth & development*

Substances

  • Culture Media
  • Acetylglucosamine