Sustained systemic delivery of monoclonal antibodies by genetically modified skin fibroblasts

J Invest Dermatol. 2000 Oct;115(4):740-5. doi: 10.1046/j.1523-1747.2000.00106.x.

Abstract

In vivo production and systemic delivery of therapeutic antibodies by engineered cells might advantageously replace injection of purified antibodies for treating a variety of life-threatening diseases, including cancer, acquired immunodeficiency syndrome, and autoimmune diseases. We report here that skin fibroblasts retrovirally transduced to express immunoglobulin genes can be used for sustained long-term systemic delivery of cloned antibodies in immunocompetent mice. Importantly, no anti- idiotypic response against the ectopically expressed model antibody used in this study was observed. This supports the notion that skin fibroblasts can potentially be used in antibody-based gene/cell therapy protocols without inducing any adverse immune response in treated individuals.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antibodies, Monoclonal / genetics*
  • Antibodies, Monoclonal / therapeutic use*
  • Antibody Formation / genetics
  • Disease Models, Animal
  • Fibroblasts / immunology*
  • Genetic Therapy
  • Humans
  • Immunocompetence
  • Mice / immunology*
  • Skin / cytology*

Substances

  • Antibodies, Monoclonal