Inhibition of T-lymphocyte proliferation by cucurbitacins from Picrorhiza scrophulariaeflora

H F Smit; A J van den Berg; B H Kroes; C J Beukelman; H C Quarles van Ufford; H van Dijk; R P Labadie

doi:10.1021/np990215q

Inhibition of T-lymphocyte proliferation by cucurbitacins from Picrorhiza scrophulariaeflora

J Nat Prod. 2000 Sep;63(9):1300-2. doi: 10.1021/np990215q.

Authors

H F Smit¹, A J van den Berg, B H Kroes, C J Beukelman, H C Quarles van Ufford, H van Dijk, R P Labadie

Affiliation

¹ Section Medicinal Chemistry, Department of Pharmacy, Universiteit Utrecht, P.O. Box 80082, 3508 TB Utrecht, The Netherlands. [email protected]

PMID: 11000045
DOI: 10.1021/np990215q

Abstract

Two cucurbitacin aglycons were isolated from the dried rhizomes of Picrorhiza scrophulariaeflora and were identified as 25-acetoxy-2,3, 16,20-tetrahydroxy-9-methyl-19-norlanosta-5,23-dien-22-one (picracin, 1) and 2,3,16,20,25-pentahydroxy-9-methyl-19-norlanosta-5, 23-dien-22-one (deacetylpicracin, 2). Both compounds inhibit mitogen-induced T-lymphocyte proliferation at an IC(50) value of 1 microM.

MeSH terms

Anti-Inflammatory Agents, Non-Steroidal / chemistry
Anti-Inflammatory Agents, Non-Steroidal / isolation & purification
Anti-Inflammatory Agents, Non-Steroidal / pharmacology*
Cell Division / drug effects
Humans
In Vitro Techniques
Magnoliopsida
Molecular Structure
Plants, Medicinal / chemistry*
Spectrum Analysis
T-Lymphocytes / cytology
T-Lymphocytes / drug effects*
Triterpenes / chemistry
Triterpenes / isolation & purification
Triterpenes / pharmacology*

Substances

Anti-Inflammatory Agents, Non-Steroidal
Triterpenes