Abstract
Human immunodeficiency virus type 1 (HIV-1) virion is known to carry a number of cellular components including cellular topoisomerase I. Previously, we have demonstrated that topoisomerase I enhances HIV-1 cDNA synthesis in reverse transcription (RT) assays in vitro. In the present study, we have produced six monoclonal antibodies (MAbs) against human topoisomerase I. The MAbs suppressed nicking/closing of supercoiled DNA and cDNA synthesis in an endogenous reverse transcription (ERT) assay using a detergent-disrupted HIV-1 virion. Thus, the results suggest that topoisomerase I plays an important role in RNA-directed DNA polymerization.
Publication types
-
Research Support, Non-U.S. Gov't
MeSH terms
-
Animals
-
Antibodies, Monoclonal / pharmacology*
-
Antibody Specificity / immunology
-
Blotting, Southern
-
Cell Line
-
DNA Replication / drug effects*
-
DNA Topoisomerases, Type I / immunology*
-
DNA Topoisomerases, Type I / isolation & purification
-
DNA, Complementary / biosynthesis*
-
DNA, Viral / drug effects*
-
Enzyme-Linked Immunosorbent Assay
-
Female
-
HIV-1 / drug effects*
-
HIV-1 / genetics
-
HeLa Cells
-
Humans
-
Mice
-
Mice, Inbred BALB C
-
RNA, Viral / biosynthesis
-
Reverse Transcriptase Polymerase Chain Reaction
-
Transfection
-
Virus Replication
Substances
-
Antibodies, Monoclonal
-
DNA, Complementary
-
DNA, Viral
-
RNA, Viral
-
DNA Topoisomerases, Type I