Detection of new propofol metabolites in human urine using gas chromatography/mass spectrometry and liquid chromatography/mass spectrometry techniques

Rapid Commun Mass Spectrom. 2000;14(20):1932-6. doi: 10.1002/1097-0231(20001030)14:20<1932::AID-RCM114>3.0.CO;2-P.

Abstract

Using hyphenated analytical techniques, gas chromatography/mass spectrometry (GC/MS) and liquid chromatography/mass spectrometry (LC/MS), a study on minor propofol metabolites in human urine was conducted. These techniques allowed identification of two new phase I metabolites (2-(omega-propanol)-6-isopropylphenol and 2-(omega-propanol)-6-isopropyl-1,4-quinol). In addition, their four corresponding conjugates (three glucuronides and one sulphate) were detected. Thus in human urine at least eight conjugate metabolites are produced, derived from four different aglycones (propofol; 2, 6-diisopropyl-1,4-quinol; 2-(omega-propanol)-6-isopropylphenol and 2-(omega-propanol)-6-isopropyl-1,4-quinol).

MeSH terms

  • Anesthetics, Intravenous / pharmacokinetics
  • Anesthetics, Intravenous / urine*
  • Biotransformation
  • Chromatography, High Pressure Liquid
  • Chromatography, Liquid
  • Gas Chromatography-Mass Spectrometry
  • Humans
  • Indicators and Reagents
  • Mass Spectrometry
  • Propofol / pharmacokinetics
  • Propofol / urine*

Substances

  • Anesthetics, Intravenous
  • Indicators and Reagents
  • Propofol