Increased leukocyte aggregation induced by gamma-globulin: a clue to the presence of pseudoleukopenia

Am J Med Sci. 2000 Sep;320(3):177-82. doi: 10.1097/00000441-200009000-00008.

Abstract

Background: Previous articles have reported the appearance of leukopenia after the administration of high-dose intravenous gamma-globulins.

Methods: A simple slide test was used to reveal the state of leukocyte aggregation (LA) in the peripheral blood as well as the absolute number of leukocytes in the slides.

Results: A significant (P < 0.00001) increment was noted in the state of LA, from 9 +/- 6% before to 25 +/- 11% after gamma-globulin administration. This was accompanied by a significant (P < 0.0004) reduction in the number of white blood cells in the peripheral blood (from 8433 +/- 3905 to 6550 +/- 3252 cells/mm3) but no significant change in the absolute number of the cells as determined by the leukocyte count per high power field in the peripheral slides.

Conclusions: We raise the possibility that the leukopenia reported in some patients after the intravenous administration of high dose gamma-globulin is explained in part by the agglutination effect of the drug.

Publication types

  • Clinical Trial

MeSH terms

  • Adolescent
  • Adult
  • Aged
  • Artifacts
  • Cell Aggregation / drug effects*
  • Female
  • Fibrinogen / metabolism
  • Humans
  • Immunoglobulins, Intravenous / administration & dosage
  • Immunoglobulins, Intravenous / pharmacology
  • Immunoglobulins, Intravenous / therapeutic use
  • Leukocyte Count / drug effects
  • Leukocytes / drug effects
  • Leukocytes / pathology*
  • Leukopenia / diagnosis*
  • Leukopenia / pathology
  • Male
  • Middle Aged
  • Reproducibility of Results
  • gamma-Globulins / administration & dosage
  • gamma-Globulins / pharmacology*
  • gamma-Globulins / therapeutic use

Substances

  • Immunoglobulins, Intravenous
  • gamma-Globulins
  • Fibrinogen