Structure, expression, and chromosomal localization of the human gene encoding a germinal center-associated nuclear protein (GANP) that associates with MCM3 involved in the initiation of DNA replication

Gene. 2000 Sep 19;255(2):219-27. doi: 10.1016/s0378-1119(00)00336-x.

Abstract

A 210kDa protein named GANP is upregulated in germinal center (GC)-B cells in the spleen of antigen-immunized mouse. We studied a human ganp gene (hganp) encoding a putative polypeptide of 1980 amino acids. The carboxyl-terminal 721-amino-acid sequence of hGANP is identical to Map80, that is presumably generated by alternative splicing of hganp/Map80 gene. The genomic segment carrying hganp and Map80 genes was isolated, and the chromosomal location was determined on 21q22.3. Northern blot analysis with RNAs from various organs demonstrated a single band of 7kb hganp mRNA, which suggests a preferential transcription of hganp gene from the hganp/Map80 locus. The hGANP expression was upregulated in GCs of the tonsil, as demonstrated by in-situ RNA hybridization and immunohistochemical analyses. The hGANP, with the domain (Map-box) capable of binding to MCM3 in B cells, might be involved in regulation of cell-cycle progression and DNA replication of GC-B cells.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Acetyltransferases*
  • Amino Acid Sequence
  • B-Lymphocytes / cytology
  • B-Lymphocytes / metabolism
  • Base Sequence
  • Blotting, Northern
  • Cell Cycle Proteins / metabolism*
  • Chromosome Mapping
  • Chromosomes, Human, Pair 21 / genetics
  • Cloning, Molecular
  • DNA Replication*
  • DNA, Complementary / chemistry
  • DNA, Complementary / genetics
  • DNA-Binding Proteins
  • Female
  • Gene Expression
  • Genes / genetics
  • Germinal Center / metabolism*
  • Humans
  • Immunohistochemistry
  • In Situ Hybridization
  • In Situ Hybridization, Fluorescence
  • Intracellular Signaling Peptides and Proteins
  • Minichromosome Maintenance Complex Component 3
  • Molecular Sequence Data
  • Nuclear Proteins / genetics*
  • Nuclear Proteins / metabolism
  • Phosphoproteins / genetics*
  • Phosphoproteins / metabolism
  • RNA, Messenger / genetics
  • RNA, Messenger / metabolism
  • Sequence Alignment
  • Sequence Analysis, DNA
  • Sequence Homology, Nucleic Acid
  • Tissue Distribution
  • Tumor Cells, Cultured

Substances

  • Cell Cycle Proteins
  • DNA, Complementary
  • DNA-Binding Proteins
  • Intracellular Signaling Peptides and Proteins
  • MCM3 protein, human
  • Mcm3 protein, mouse
  • Nuclear Proteins
  • Phosphoproteins
  • RNA, Messenger
  • Acetyltransferases
  • MCM3AP protein, human
  • Minichromosome Maintenance Complex Component 3

Associated data

  • GENBANK/AJ010089