Enhanced p73 expression during differentiation and complex p73 isoforms in myeloid leukemia

M P Tschan; T J Grob; U R Peters; V D Laurenzi; B Huegli; K A Kreuzer; C A Schmidt; G Melino; M F Fey; A Tobler; J F Cajot

doi:10.1006/bbrc.2000.3627

Enhanced p73 expression during differentiation and complex p73 isoforms in myeloid leukemia

Biochem Biophys Res Commun. 2000 Oct 14;277(1):62-5. doi: 10.1006/bbrc.2000.3627.

Authors

M P Tschan¹, T J Grob, U R Peters, V D Laurenzi, B Huegli, K A Kreuzer, C A Schmidt, G Melino, M F Fey, A Tobler, J F Cajot

Affiliation

¹ Department of Clinical Research, Institute of Medical Oncology, Bern, Switzerland.

PMID: 11027640
DOI: 10.1006/bbrc.2000.3627

Abstract

The p53 homologue p73 is expressed in at least six different isoforms (alpha, beta, gamma, delta, epsilon, and zeta), but unlike p53 it has rarely been found mutated in human cancers. However, altered expression of this gene has been reported in cancer cells. In order to understand if p73 is involved in normal and malignant development of myeloid cells, we investigated the expression pattern of the different p73 isoforms in progenitor and mature normal myeloid cells as well as in cells derived from acute and chronic myeloid leukemias. The results show that expression of p73 is markedly enhanced during differentiation of myeloid leukemic cells and that leukemic blasts from patients show an increased expression of the shorter p73 isoforms (gamma, delta, epsilon, zeta). In particular the epsilon isoform is only expressed in leukemic cells and completely absent in mature myeloid cells. Altogether our data suggest that p73 is involved in myeloid differentiation and its altered expression is involved in leukemic degeneration.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Acute Disease
Blotting, Southern
Blotting, Western
Cell Differentiation*
DNA-Binding Proteins / genetics*
DNA-Binding Proteins / metabolism
Gene Expression Regulation, Neoplastic*
Genes, Tumor Suppressor
HL-60 Cells
Humans
Leukemia, Myelogenous, Chronic, BCR-ABL Positive / genetics
Leukemia, Myelogenous, Chronic, BCR-ABL Positive / metabolism
Leukemia, Myelogenous, Chronic, BCR-ABL Positive / pathology
Leukemia, Myeloid / genetics
Leukemia, Myeloid / metabolism*
Leukemia, Myeloid / pathology*
Myeloid Cells / cytology
Myeloid Cells / metabolism
Myeloid Progenitor Cells / cytology
Myeloid Progenitor Cells / metabolism
Nuclear Proteins / genetics*
Nuclear Proteins / metabolism
Protein Isoforms / genetics
Protein Isoforms / metabolism
RNA, Messenger / genetics
RNA, Messenger / metabolism
Reverse Transcriptase Polymerase Chain Reaction
Tumor Cells, Cultured
Tumor Protein p73
Tumor Suppressor Proteins

Substances

DNA-Binding Proteins
Nuclear Proteins
Protein Isoforms
RNA, Messenger
TP73 protein, human
Tumor Protein p73
Tumor Suppressor Proteins