An immunoglobulin G based chimeric protein induced foot-and-mouth disease specific immune response in swine

Vaccine. 2000 Oct 15;19(4-5):538-46. doi: 10.1016/s0264-410x(00)00186-9.

Abstract

Epitopes containing the residues 141aa-160aa and 200aa-213aa from foot-and-mouth disease (FMD) serotype O1K HK type FMDV VP1 were joined to a swine immunoglobulin G single heavy chain constant region (scIgG), creating a novel chimeric protein, named F1-scIgG. In this study, inoculation with F1-scIgG induced both FMD virus-neutralizing antibody response and T cell response in swine. Antisera from these F1-scIgG-inoculated swine protected suckling mice against 1000 lethal dose 50 (1000LD(50)) FMD challenge. F1-scIgG-inoculated swine were also fully protected against 50LD(50) FMD virus challenge. The present study demonstrates the clear potential for viral epitopes linked with self-Ig in novel FMD vaccine design.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antibodies, Viral / biosynthesis
  • Antigens, Viral / chemistry
  • Antigens, Viral / genetics
  • Aphthovirus / genetics
  • Aphthovirus / immunology*
  • Base Sequence
  • DNA Primers / genetics
  • Epitopes / chemistry
  • Epitopes / genetics
  • Foot-and-Mouth Disease / prevention & control
  • Immunoglobulin G / chemistry
  • Immunoglobulin G / genetics
  • Immunoglobulin G / immunology*
  • In Vitro Techniques
  • Lymphocyte Activation
  • Mice
  • Neutralization Tests
  • Recombinant Fusion Proteins / chemistry
  • Recombinant Fusion Proteins / genetics
  • Recombinant Fusion Proteins / immunology
  • Swine
  • T-Lymphocytes / immunology
  • Viral Vaccines / genetics
  • Viral Vaccines / immunology
  • Viral Vaccines / pharmacology

Substances

  • Antibodies, Viral
  • Antigens, Viral
  • DNA Primers
  • Epitopes
  • Immunoglobulin G
  • Recombinant Fusion Proteins
  • Viral Vaccines