Abstract
The Ly-6 locus encodes several cell surface proteins of 10-12 kDa. Some members of this multigene family may function in cell signaling and/or cell adhesion processes. T lymphocytes overexpressing Ly-6A.2 (one member of the Ly-6 gene family) protein homotypically aggregate when cultured in vitro. Further analysis of this homotypic aggregation suggests that Ly-6A.2 participates in cell-cell adhesion. These observations indicated the presence of a Ly-6 ligand(s) on the surface of lymphoid cells. In this study we report generation of a hamster mAb, 9AB2, that blocks Ly-6A.2-dependent cell-cell adhesion. The 9AB2 Ab recognizes a 66-kDa glycoprotein with unique tissue expression. The 9AB2 mAb does not bind Ly-6A.2, but coimmunoprecipitates Ly-6A.2 molecule. Moreover, 9AB2 Ag-expressing thymocytes specifically bind to Chinese hamster ovary cells overexpressing Ly-6A.2 protein, and this binding is specifically blocked by 9AB2 and anti-Ly-6A.2 Abs. These results suggest that the 66-kDa protein recognized by 9AB2 mAb is the putative ligand for Ly-6A.2.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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3T3 Cells
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Animals
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Antibodies, Blocking / biosynthesis
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Antibodies, Blocking / metabolism
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Antibodies, Blocking / pharmacology
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Antibodies, Monoclonal / biosynthesis
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Antibodies, Monoclonal / metabolism
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Antibodies, Monoclonal / pharmacology*
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Antigens, Ly / physiology*
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Binding Sites, Antibody
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CHO Cells
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Cell Adhesion / immunology
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Cell Aggregation / immunology
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Cell Line
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Cell Line, Transformed
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Cricetinae
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Humans
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Immunosuppressive Agents / pharmacology*
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Ligands
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Membrane Proteins / antagonists & inhibitors*
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Membrane Proteins / physiology*
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Mice
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Mice, Inbred BALB C
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Mice, Transgenic
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Molecular Weight
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Organ Specificity / genetics
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Organ Specificity / immunology
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Protein Biosynthesis*
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Proteins / immunology*
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Proteins / isolation & purification
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Proteins / metabolism
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Spleen / immunology*
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Spleen / metabolism
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T-Lymphocytes / immunology
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T-Lymphocytes / metabolism
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Thymus Gland / cytology
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Thymus Gland / immunology*
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Thymus Gland / metabolism
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Transgenes / immunology
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Tumor Cells, Cultured
Substances
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Antibodies, Blocking
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Antibodies, Monoclonal
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Antigens, Ly
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Immunosuppressive Agents
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Ligands
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Ly6a protein, mouse
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Membrane Proteins
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Proteins