Identification of bacterial class I accessible proteins by disseminated insertion of class I epitopes

Methods Enzymol. 2000:326:516-27. doi: 10.1016/s0076-6879(00)26073-4.

Authors

D Ellefson¹, A W van der Velden, D Parker, F Heffron

Affiliation

¹ Department of Molecular Microbiology and Immunology, Oregon Health Sciences University, Portland 97201, USA.

PMID: 11036661
DOI: 10.1016/s0076-6879(00)26073-4

No abstract available

MeSH terms

Amino Acid Sequence
Animals
Artificial Gene Fusion / methods
Bacterial Proteins / genetics*
Bone Marrow Cells / cytology
Chromosomes, Bacterial
DNA Transposable Elements
Epitopes / genetics*
Escherichia coli / genetics
Flow Cytometry / methods
Genetic Vectors
Humans
Macrophages / cytology
Macrophages / physiology
Mice
Mice, Inbred C57BL
Molecular Sequence Data
Mutagenesis, Insertional
Plasmids
Recombinant Fusion Proteins / analysis
Recombinant Fusion Proteins / genetics
Salmonella typhimurium / genetics
Transposases / genetics

Substances

Bacterial Proteins
DNA Transposable Elements
Epitopes
Recombinant Fusion Proteins
Transposases