cDNA cloning, chromosomal mapping, and expression analysis of human VE-Cadherin-2

Mamm Genome. 2000 Nov;11(11):1030-3. doi: 10.1007/s003350010186.

Abstract

Murine vascular endothelial cadherin-2 (VE-cad-2) is a cellular adhesion molecule that is distinct from vascular endothelial cadherin 1 (VE-cad-1) in that it does not interact with catenins and does not appear to affect cell migration or growth. In this study, we have cloned a full-length cDNA of the human homolog of VE-cad-2 and used it to map the chromosomal locus of the VE-cad-2 gene. Human VE-cad-2 maps to Chromosome (Chr) 5q31. The cDNA of human VE-cad-2 is highly homologous to mouse VE-cad-2, except for a C-terminal tail. The genomic structure of VE-cad-2 is strikingly similar to that reported for a large family of neuronal protocadherin genes mapped to Chr 5q, yet the amino acid sequences between VE-cad-2 and the protocadherins are substantially divergent. The promoter of human VE-cad-2 contains two TATA boxes and transcription initiates from a single site 3' to these elements. Similar to mouse VE-cad-2, the human gene is expressed primarily in highly vascularized tissues.

MeSH terms

  • 5' Untranslated Regions
  • Amino Acid Sequence
  • Base Sequence
  • Cadherins / genetics*
  • Cadherins / metabolism*
  • Chromosome Mapping
  • Chromosomes, Human, Pair 5*
  • Cloning, Molecular
  • DNA, Complementary
  • Endothelium, Vascular / physiology*
  • Exons
  • Humans
  • Molecular Sequence Data
  • Promoter Regions, Genetic
  • Protocadherins
  • TATA Box

Substances

  • 5' Untranslated Regions
  • Cadherins
  • DNA, Complementary
  • PCDH12 protein, human
  • Protocadherins

Associated data

  • GENBANK/AF240635