Abstract
Dendritic spines receive most excitatory inputs in the CNS and compartmentalize calcium. Spines also undergo rapid morphological changes, although the function of this motility is still unclear. We have investigated the effect of spine movement on spine calcium dynamics with two-photon photobleaching of enhanced green fluorescent protein and calcium imaging of action potential-elicited transients in spines from layer 2/3 pyramidal neurons in mouse visual cortex slices. The elongation or retraction of the spine neck during spine motility alters the diffusional coupling between spine and dendrite and significantly changes calcium decay kinetics in spines. Our results demonstrate that the spine's ability to compartmentalize calcium is constantly changing.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Acoustic Stimulation
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Action Potentials / physiology
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Animals
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Calcium / metabolism*
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Cell Compartmentation / physiology
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Cell Movement / physiology*
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Cells, Cultured
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Dendrites / metabolism*
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Dendrites / ultrastructure
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Diffusion
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Fluorescent Dyes
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Green Fluorescent Proteins
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In Vitro Techniques
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Luminescent Proteins / genetics
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Luminescent Proteins / metabolism
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Mice
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Mice, Inbred C57BL
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Microinjections
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Microscopy, Fluorescence
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Patch-Clamp Techniques
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Photochemistry
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Pyramidal Cells / metabolism*
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Pyramidal Cells / ultrastructure
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Transfection
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Visual Cortex / cytology
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Visual Cortex / metabolism*
Substances
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Fluorescent Dyes
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Luminescent Proteins
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Green Fluorescent Proteins
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Calcium