Use of molecular beacons to detect an antifolate resistance-associated mutation in Plasmodium falciparum

R Durand; J Eslahpazire; S Jafari; J F Delabre; A Marmorat-Khuong; J P di Piazza; J Le Bras

doi:10.1128/AAC.44.12.3461-3464.2000

Use of molecular beacons to detect an antifolate resistance-associated mutation in Plasmodium falciparum

Antimicrob Agents Chemother. 2000 Dec;44(12):3461-4. doi: 10.1128/AAC.44.12.3461-3464.2000.

Authors

R Durand¹, J Eslahpazire, S Jafari, J F Delabre, A Marmorat-Khuong, J P di Piazza, J Le Bras

Affiliation

¹ Centre National de Référence pour la Chimiosensibilité du Paludisme, Université Paris V et Assistance Publique-Hôpitaux de Paris, Hôpital Bichat-Claude Bernard, Laboratoire de Parasitologie, Paris, France. [email protected]

Abstract

A PCR-based technique using new fluorescent probes, called molecular beacons, was developed to detect the antifolate resistance-associated S108N point mutation in Plasmodium falciparum. One hundred African clinical isolates were tested by the new method in comparison with the PCR-restriction fragment length polymorphism method. This new molecular technique appears to be a promising tool for epidemiological studies.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Drug Resistance / genetics
Folic Acid Antagonists / pharmacology*
Humans
Mutation
Plasmodium falciparum / drug effects
Plasmodium falciparum / enzymology
Plasmodium falciparum / genetics*
Polymerase Chain Reaction / methods
Polymorphism, Restriction Fragment Length
Tetrahydrofolate Dehydrogenase / genetics*

Substances

Folic Acid Antagonists
Tetrahydrofolate Dehydrogenase