Short-term exposure to membrane-active antibiotics inhibits Cryptosporidium parvum infection in cell culture

Antimicrob Agents Chemother. 2000 Dec;44(12):3473-5. doi: 10.1128/AAC.44.12.3473-3475.2000.

Abstract

A cell culture system and double fluorogenic staining were used to study the susceptibility of Cryptosporidium parvum to membrane-active antibiotics. Buforin II and magainin II exerted a cytotoxic effect on sporozoites but did not consistently affect oocyst viability. Lasalocid and nigericin demonstrated less activity against sporozoites but reduced the infectivity of oocysts.

MeSH terms

  • Animals
  • Anti-Infective Agents / pharmacology*
  • Antimicrobial Cationic Peptides / pharmacology*
  • Cell Membrane / drug effects
  • Cryptosporidium parvum / cytology
  • Cryptosporidium parvum / drug effects*
  • Drug Evaluation, Preclinical
  • Flow Cytometry
  • Fluorescent Dyes
  • Humans
  • Magainins
  • Proteins / pharmacology*
  • Time Factors
  • Tumor Cells, Cultured / drug effects
  • Tumor Cells, Cultured / parasitology
  • Xenopus Proteins*

Substances

  • Anti-Infective Agents
  • Antimicrobial Cationic Peptides
  • Fluorescent Dyes
  • Magainins
  • Proteins
  • Xenopus Proteins
  • buforin II
  • magainin 2 peptide, Xenopus