Abstract
Immunization of mice with the heat shock protein (HSP) gp96 but not control proteins leads to 5- to 7-fold enlargement of draining lymph nodes (LNs) resulting from accumulation of large numbers of mature CD11c(+) cells, but not T or B lymphocytes in them. The increase in size and cellularity is time-dependent; the draining LNs reach their peak size between 12 and 24 h after injection and regress to their normal size between 48 and 72 h after injection. The increment is elicited specifically in the draining LN but not in other LNs. This observation uncovers a novel aspect of HSP-APC interaction and adds to the mechanistic explanation for the unusually high immunogenicity of HSP-peptide complexes.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Animals
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Antigen-Presenting Cells / cytology
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Antigen-Presenting Cells / immunology
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Antigen-Presenting Cells / metabolism
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Antigens, Neoplasm / administration & dosage*
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Antigens, Neoplasm / immunology
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Antigens, Neoplasm / physiology*
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Cell Differentiation / immunology
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Cell Division / immunology
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Cell Movement / immunology*
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Growth Substances / administration & dosage*
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Growth Substances / immunology
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Growth Substances / physiology*
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Heat-Shock Proteins / administration & dosage*
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Heat-Shock Proteins / immunology
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Heat-Shock Proteins / physiology*
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Injections, Intradermal
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Integrin alphaXbeta2 / biosynthesis*
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Lymph Nodes / anatomy & histology
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Lymph Nodes / cytology
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Lymph Nodes / immunology
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Lymph Nodes / metabolism
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Mice
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Mice, Inbred C57BL
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Organ Size / immunology
Substances
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Antigens, Neoplasm
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Growth Substances
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Heat-Shock Proteins
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Integrin alphaXbeta2
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sarcoma glycoprotein gp96 rejection antigens