Abstract
The structure of a gene coding for bombinin-like peptides (BLP) in Bombina orientalis was determined. It comprises two exons separated by a 1337 bp intron. Exon 1 codes for the signal peptide, while exon 2 contains the genetic information for BLP-7 and a bombinin H-type peptide (GH-2). The promoter region contains putative recognition sites for nuclear factors, such as NF-IL6 and NF-kappaB. The analysis of the structure of this gene, compared with that of the previously reported BLP-3 gene sequence, suggests the occurrence of a gene duplication event, rather than an alternative splicing mechanism, which leads to the generation of both inter- and intra-families variability in this class of cytolytic peptides. Furthermore, chromosome walking analysis indicates that this gene family is not densely clustered.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Amphibian Proteins*
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Animals
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Anti-Infective Agents / chemistry*
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Antimicrobial Cationic Peptides / chemistry
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Antimicrobial Cationic Peptides / genetics*
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Anura
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Base Sequence
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Binding Sites
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Blotting, Southern
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CCAAT-Enhancer-Binding Protein-beta / genetics
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Chromosome Walking
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Exons
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Introns
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Models, Genetic
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Molecular Sequence Data
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NF-kappa B / genetics
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Peptides / chemistry
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Peptides / genetics*
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Promoter Regions, Genetic
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Protein Sorting Signals
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Sequence Analysis, DNA
Substances
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Amphibian Proteins
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Anti-Infective Agents
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Antimicrobial Cationic Peptides
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BLP-7 protein, Bombina orientalis
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CCAAT-Enhancer-Binding Protein-beta
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NF-kappa B
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Peptides
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Protein Sorting Signals
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bombinin-like peptide-2, Bombina orientalis
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bombinin-like peptide-3, Bombina orientalis