Protein production: feeding the crystallographers and NMR spectroscopists

Nat Struct Biol. 2000 Nov:7 Suppl:970-2. doi: 10.1038/80751.

Abstract

Protein purification efforts for structural genomics will focus on automation for the readily-expressed proteins, and process development for the more difficult ones, such as membrane proteins. Thousands of proteins are expected to be produced in the next few years. The purified proteins will be valuable reagents for the entire research community.

Publication types

  • Research Support, Non-U.S. Gov't
  • Review

MeSH terms

  • Animals
  • Cloning, Molecular
  • Crystallography, X-Ray*
  • Gene Expression
  • Membrane Proteins / biosynthesis
  • Membrane Proteins / chemistry
  • Membrane Proteins / genetics
  • Membrane Proteins / isolation & purification
  • Nuclear Magnetic Resonance, Biomolecular*
  • Protein Biosynthesis*
  • Protein Structure, Tertiary
  • Proteins / chemistry*
  • Proteins / genetics
  • Proteins / isolation & purification
  • Quality Control
  • Recombinant Fusion Proteins / biosynthesis
  • Recombinant Fusion Proteins / chemistry
  • Recombinant Fusion Proteins / isolation & purification
  • Recombinant Fusion Proteins / standards
  • Robotics

Substances

  • Membrane Proteins
  • Proteins
  • Recombinant Fusion Proteins