Effects of lipoxygenase metabolites of arachidonic acid on the growth of human blood CD34(+) progenitors

Blood Cells Mol Dis. 2000 Oct;26(5):427-36. doi: 10.1006/bcmd.2000.0321.

Abstract

The influence of lipoxygenase metabolites of arachidonic acid on proliferation and differentiation of CD34(+) cells was studied. Their effects on the CFU-GM and BFU-E progenitors were investigated by culture of CD34(+) cells in liquid or semisolid medium. Only 12-HETE (1 microM) stimulated the [(3)H]thymidine as well as BrdU incorporation and increased the number of cell divisions (PKH2 tracking). Addition of 12-HETE and 15-HETE but not of LXA(4), LXB(4), LTB(4), and LTC(4) to liquid cultures of CD34(+) cells for 3 and 8 days reduced in a time-dependent manner the number of CFU-GM and BFU-E. Both HETEs also increased the percentage of glycophorin A(+) cells while they reduced the percentage of CD34(-)/CD33(+) cells after 3 and 5 days of liquid cultures. These results show that HETE treatment stimulates proliferation and accelerates the differentiation of CD34(+) cells, mostly toward the erythroid lineage.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • 12-Hydroxy-5,8,10,14-eicosatetraenoic Acid / pharmacology
  • Antigens, CD34 / analysis*
  • Antigens, Differentiation / biosynthesis
  • Antigens, Differentiation / drug effects
  • Arachidonic Acids / metabolism
  • Arachidonic Acids / pharmacology*
  • Cell Count
  • Cell Division / drug effects*
  • Colony-Forming Units Assay
  • Culture Media / pharmacology
  • Dose-Response Relationship, Drug
  • Erythroid Precursor Cells / cytology
  • Erythroid Precursor Cells / drug effects
  • Erythropoietin / pharmacology
  • Hematopoietic Stem Cells / chemistry
  • Hematopoietic Stem Cells / cytology
  • Hematopoietic Stem Cells / drug effects*
  • Humans
  • Hydroxyeicosatetraenoic Acids / pharmacology
  • Leukotriene B4 / pharmacology
  • Lipoxins*
  • Lipoxygenase / metabolism*
  • Methylcellulose
  • Solutions
  • Thymidine / metabolism
  • Time Factors

Substances

  • Antigens, CD34
  • Antigens, Differentiation
  • Arachidonic Acids
  • Culture Media
  • Hydroxyeicosatetraenoic Acids
  • Lipoxins
  • Solutions
  • lipoxin A4
  • Erythropoietin
  • Leukotriene B4
  • 12-Hydroxy-5,8,10,14-eicosatetraenoic Acid
  • 15-hydroxy-5,8,11,13-eicosatetraenoic acid
  • Methylcellulose
  • lipoxin B4
  • Lipoxygenase
  • Thymidine