Abstract
The mouse Otx2 gene is a homeobox transcription factor required as early as gastrulation for the proper development of the head. We compared gene expression profiles in wild-type and Otx2(-/-) 6.5 days postcoitum embryos by using a serial analysis of gene expression assay adapted to microdissected structures. Among a broader list, the study of six genes found to be differentially expressed allows defining a role for Otx2 in the orchestration of cell movements leading to the adequate organization of the embryo before gastrulation.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Cystatin B
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Cystatins / genetics
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Cytokines
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Ectoderm
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Embryonic and Fetal Development
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Endoderm
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Expressed Sequence Tags
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Female
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Fibroblast Growth Factors / genetics
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Gastrula / physiology*
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Gene Expression Profiling*
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Genotype
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Head / embryology*
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Homeodomain Proteins / genetics
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Homeodomain Proteins / physiology*
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Humans
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In Situ Hybridization
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Intercellular Signaling Peptides and Proteins
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Male
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Mice
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Mice, Knockout
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Nerve Tissue Proteins / genetics
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Nerve Tissue Proteins / physiology*
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Nodal Protein
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Otx Transcription Factors
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Polycomb Repressive Complex 2
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Proteins / genetics
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Proto-Oncogene Proteins / genetics
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Repressor Proteins / genetics
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Repressor Proteins / physiology
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Trans-Activators / genetics
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Trans-Activators / physiology*
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Transcription Factors
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Transforming Growth Factor beta / genetics
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Wnt Proteins
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Wnt4 Protein
Substances
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CSTB protein, human
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Cer1 protein, mouse
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Cstb protein, mouse
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Cystatins
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Cytokines
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DKK1 protein, human
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Dkk1 protein, mouse
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EED protein, human
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Eed protein, mouse
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HHEX protein, human
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Hhex protein, mouse
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Homeodomain Proteins
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Intercellular Signaling Peptides and Proteins
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NODAL protein, human
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Nerve Tissue Proteins
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Nodal Protein
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Nodal protein, mouse
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OTX2 protein, human
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Otx Transcription Factors
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Otx2 protein, mouse
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Proteins
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Proto-Oncogene Proteins
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Repressor Proteins
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Trans-Activators
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Transcription Factors
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Transforming Growth Factor beta
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WNT4 protein, human
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Wnt Proteins
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Wnt4 Protein
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Wnt4 protein, mouse
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fibroblast growth factor 15, mouse
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Fibroblast Growth Factors
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Cystatin B
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Polycomb Repressive Complex 2