Abstract
Like most organisms, plants have endogenous biological clocks that coordinate internal events with the external environment. We used high-density oligonucleotide microarrays to examine gene expression in Arabidopsis and found that 6% of the more than 8000 genes on the array exhibited circadian changes in steady-state messenger RNA levels. Clusters of circadian-regulated genes were found in pathways involved in plant responses to light and other key metabolic pathways. Computational analysis of cycling genes allowed the identification of a highly conserved promoter motif that we found to be required for circadian control of gene expression. Our study presents a comprehensive view of the temporal compartmentalization of physiological pathways by the circadian clock in a eukaryote.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, Non-P.H.S.
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Arabidopsis / genetics
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Arabidopsis / growth & development
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Arabidopsis / physiology*
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Biological Clocks / genetics*
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Carbohydrate Metabolism
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Circadian Rhythm*
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Gene Expression Profiling
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Gene Expression Regulation, Plant*
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Genes, Plant
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Light
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Nitrogen / metabolism
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Oligonucleotide Array Sequence Analysis
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Photosynthesis / genetics
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Photosynthetic Reaction Center Complex Proteins / genetics
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Plants, Genetically Modified
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Promoter Regions, Genetic
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RNA, Messenger / genetics
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RNA, Messenger / metabolism
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RNA, Plant / genetics
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RNA, Plant / metabolism
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Sulfur / metabolism
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Transcription, Genetic*
Substances
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Photosynthetic Reaction Center Complex Proteins
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RNA, Messenger
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RNA, Plant
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Sulfur
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Nitrogen