Abstract
Two periwinkle cDNAs (crdxr and crmecs) encoding enzymes of the non-mevalonate terpenoid pathway were characterized using reverse transcription-PCR strategy based on the design of degenerated oligonucleotides. The deduced amino acid sequence of crdxr is homologue to 1-deoxy-D-xylulose 5-phosphate reductoisomerases. Crmecs represents the first plant cDNA encoding a protein similar to the 2C-methyl-D-erythritol 2,4-cyclodiphosphate synthase from Escherichia coli. Expression of crdxr and crmecs genes was up-regulated in periwinkle cells producing monoterpenoid indole alkaloids. Involvement of the 2C-methyl-D-erythritol 4-phosphate pathway in alkaloid biosynthesis is discussed.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Aldose-Ketose Isomerases / chemistry
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Aldose-Ketose Isomerases / genetics*
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Alkaloids
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Amino Acid Sequence
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Base Sequence
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Cloning, Molecular
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DNA, Complementary / chemistry*
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Erythritol / analogs & derivatives
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Erythritol / metabolism*
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Genes, Plant*
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Molecular Sequence Data
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Multienzyme Complexes / chemistry
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Multienzyme Complexes / genetics*
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Nucleotidyltransferases / chemistry
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Nucleotidyltransferases / genetics*
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Oxidoreductases / chemistry
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Oxidoreductases / genetics*
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Reverse Transcriptase Polymerase Chain Reaction
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Sequence Alignment
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Sequence Homology, Amino Acid
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Sugar Phosphates / metabolism*
Substances
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2-C-methylerythritol 4-phosphate
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Alkaloids
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DNA, Complementary
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Multienzyme Complexes
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Sugar Phosphates
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Oxidoreductases
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1-deoxy-D-xylulose 5-phosphate reductoisomerase
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4-diphosphocytidyl-2-C--methylerythritol synthase
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Nucleotidyltransferases
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Aldose-Ketose Isomerases
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Erythritol
Associated data
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GENBANK/AF250235
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GENBANK/AF250236