COX and LOX eicosanoids modulate platelet activation and procoagulation induced by two murine cancer cells

Prostaglandins Leukot Essent Fatty Acids. 2000 Dec;63(6):377-83. doi: 10.1054/plef.2000.0228.

Abstract

Involvement of arachidonic acid cyclooxygenase (COX) and lipoxygenase (LOX) metabolites in platelet aggregation and coagulation induced by two varieties of cancer cells of murine transplantable tumors was studied. A lung alveolar carcinoma (LAC) and a fibrosarcoma (FS), induced platelet aggregation and plasma coagulation (P<0.05). Pretreatment of both tumor lines with a COX inhibitor did not block the tumor cell induced platelet aggregation (TCIPA). COX [12(S)-HTT] and LOX [12(S)-HETE], metabolites of washed platelets (WP), alone or co-incubated with LAC or FS cells, were analyzed. We observed higher 12(S)-HETE release with respect to 12(S)HHT when WP were co-incubated with LAC cells. With both neoplastic cell (NC) lines prothrombin time (PT) was shortened. Pretreatment of NC with iodoacetic acid, soybean trypsin inhibitor or Factor X-deficient plasma increased the PT. These results indicate that AA metabolites play a role on the procoagulation and platelet aggregation induced by mesenchymal and epithelial murine cancers.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adenocarcinoma, Bronchiolo-Alveolar / metabolism
  • Adenocarcinoma, Bronchiolo-Alveolar / pathology*
  • Animals
  • Arachidonic Acids / metabolism
  • Blood Coagulation / drug effects*
  • Coculture Techniques
  • Cysteine Endopeptidases / chemistry
  • Cysteine Endopeptidases / metabolism*
  • Eicosanoids / biosynthesis
  • Eicosanoids / classification
  • Eicosanoids / physiology*
  • Factor X / physiology
  • Fibrosarcoma / metabolism
  • Fibrosarcoma / pathology*
  • Iodoacetic Acid / pharmacology
  • Lipoxygenase / metabolism*
  • Lung Neoplasms / metabolism
  • Lung Neoplasms / pathology*
  • Mice
  • Mice, Inbred BALB C
  • Mice, Inbred C57BL
  • Neoplasm Proteins*
  • Platelet Activation / drug effects*
  • Prostaglandin-Endoperoxide Synthases / metabolism*
  • Thrombophilia / etiology
  • Thrombophilia / physiopathology
  • Trypsin Inhibitor, Kunitz Soybean / pharmacology
  • Tumor Cells, Cultured / drug effects
  • Tumor Cells, Cultured / metabolism

Substances

  • Arachidonic Acids
  • Eicosanoids
  • Neoplasm Proteins
  • Factor X
  • Trypsin Inhibitor, Kunitz Soybean
  • Lipoxygenase
  • Prostaglandin-Endoperoxide Synthases
  • Cysteine Endopeptidases
  • cancer procoagulant
  • Iodoacetic Acid