[Antigen presenting role of dendritic cell proliferated from peripheral blood monocytes in patients with chronic hepatitis B]

Zhonghua Gan Zang Bing Za Zhi. 2000 Dec;8(6):330-1.
[Article in Chinese]

Abstract

Objective: To study the presenting effect of dendritic cell (DC) proliferated from peripheral blood monocytes in patients with chronic hepatitis B on HBsAg.

Methods: DC proliferated from patient's peripheral blood monocytes by adding GM-CSF and IL-4 were incubated with HBsAg for 1.5h at different concentrations, then mixed with autologous T cell and cocultured in 96-well flat-bottomed microtiter plates for 5 d at 37 degrees C and 5% CO(2). (3)H-TdR was added 13h before the culture completion, and finally the cells were gathered and the cpm was tested.

Results: The proliferation of T cell by DC treated by HBsAg is significantly higher than untreated.

Conclusion: DC cultured from peripheral blood monocytes in patients with chronic hepatitis B plays a role in presenting HBsAg.

Publication types

  • English Abstract

MeSH terms

  • Antigen Presentation*
  • Dendritic Cells / physiology*
  • Hepatitis B Surface Antigens / immunology*
  • Hepatitis B, Chronic / immunology*
  • Humans
  • Lymphocyte Activation*
  • T-Lymphocytes / immunology*

Substances

  • Hepatitis B Surface Antigens