Objective: To study the relationship between genotypes of cytochrome P450IIE1 (IIE1 ) and development of chronic liver diseases.
Methods: Cytochrome P450IIE1 was genotyped by PCR and restricted endonucleases digestion of DNA prepared from peripheral white blood cells.
Results: IIE1 could be divided into three types: A, B and C based on different products digested by two endonucleases. In normal controls predominant genotypes were A and B. No relationship was found between development of non-alcoholic chronic liver diseases and IIE1 genotypes. Its genotype distribution was similar to normal controls. Detection of type A in alcoholic liver diseases was significantly decreased and type B was significantly increased (P<0.01). Detection of type A in alcohol-related hepatocellular carcinoma was also decreased and type B increased compared to non-alcohol-related hepatocellular carcinoma.
Conclusion: IIE1 genotypes play some roles in development of alcoholic liver diseases and alcohol-related hepatocellular carcinoma.