Rapid detection of enterohemorrhagic Escherichia coli by real-time PCR with fluorescent hybridization probes

T Bellin; M Pulz; A Matussek; H G Hempen; F Gunzer

doi:10.1128/JCM.39.1.370-374.2001

Rapid detection of enterohemorrhagic Escherichia coli by real-time PCR with fluorescent hybridization probes

J Clin Microbiol. 2001 Jan;39(1):370-4. doi: 10.1128/JCM.39.1.370-374.2001.

Authors

T Bellin¹, M Pulz, A Matussek, H G Hempen, F Gunzer

Affiliation

¹ Hannover Medical School, Institute of Medical Microbiology, 30625 Hannover, Germany.

Abstract

In this report, we present a PCR protocol for rapid identification of enterohemorrhagic Escherichia coli on a LightCycler instrument. In a multiplex assay, the genes encoding Shiga toxin 1 and Shiga toxin 2 are detected in a single reaction capillary. A complete analysis of up to 32 samples takes about 45 min.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Cattle
DNA Probes
DNA, Bacterial / analysis
Escherichia coli / genetics
Escherichia coli / isolation & purification*
Escherichia coli Infections / microbiology*
Feces / microbiology
Fluorescent Dyes
Humans
Meat / microbiology
Polymerase Chain Reaction / methods*
Sensitivity and Specificity
Shiga Toxin 1 / genetics
Shiga Toxin 2 / genetics

Substances

DNA Probes
DNA, Bacterial
Fluorescent Dyes
Shiga Toxin 1
Shiga Toxin 2