Synergism with the coactivator OBF-1 (OCA-B, BOB-1) is mediated by a specific POU dimer configuration

Cell. 2000 Dec 8;103(6):853-64. doi: 10.1016/s0092-8674(00)00189-6.

Abstract

POU domain proteins contain a bipartite DNA binding domain divided by a flexible linker that enables them to adopt various monomer configurations on DNA. The versatility of POU protein operation is additionally conferred at the dimerization level. The POU dimer formed on the PORE (ATTTGAAATGCAAAT) can recruit the transcriptional coactivator OBF-1, whereas POU dimers formed on the consensus MORE (ATGCATATGCAT) or on MOREs from immunoglobulin heavy chain promoters (AT[G/A][C/A]ATATGCAA) fail to interact. An interaction with OBF-1 is precluded since the same Oct-1 residues that form the MORE dimerization interface are also used for OBF-1/Oct-1 interactions on the PORE. Our findings provide a paradigm of how specific POU dimer assemblies can differentially recruit a coregulatory activity with distinct transcriptional readouts.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Motifs
  • Animals
  • Base Sequence
  • Cell Line
  • DNA-Binding Proteins / chemistry
  • DNA-Binding Proteins / genetics
  • DNA-Binding Proteins / metabolism*
  • Dimerization
  • Gene Expression Regulation
  • Genes, Reporter
  • Host Cell Factor C1
  • Humans
  • Mice
  • Models, Molecular
  • Molecular Sequence Data
  • Octamer Transcription Factor-1
  • Octamer Transcription Factor-2
  • Octamer Transcription Factor-3
  • Octamer Transcription Factor-6
  • Oligonucleotides / genetics
  • Oligonucleotides / metabolism*
  • Promoter Regions, Genetic
  • Protein Binding / genetics
  • Protein Structure, Tertiary
  • Sequence Analysis
  • Trans-Activators / genetics
  • Trans-Activators / metabolism*
  • Transcription Factor Pit-1
  • Transcription Factors / chemistry
  • Transcription Factors / genetics
  • Transcription Factors / metabolism*
  • Transcription, Genetic
  • Transfection

Substances

  • DNA-Binding Proteins
  • HCFC1 protein, human
  • Hcfc1 protein, mouse
  • Host Cell Factor C1
  • Octamer Transcription Factor-1
  • Octamer Transcription Factor-2
  • Octamer Transcription Factor-3
  • Oligonucleotides
  • POU1F1 protein, human
  • POU2AF1 protein, human
  • POU2F1 protein, human
  • POU2F2 protein, human
  • POU3F1 protein, human
  • POU5F1 protein, human
  • Pit1 protein, mouse
  • Pou2af1 protein, mouse
  • Pou2f1 protein, mouse
  • Pou2f2 protein, mouse
  • Pou3f1 protein, mouse
  • Pou5f1 protein, mouse
  • Trans-Activators
  • Transcription Factor Pit-1
  • Transcription Factors
  • Octamer Transcription Factor-6