Abstract
Here we describe a protein product of the human septin H5/PNUTL2/CDCrel2b gene, which we call ARTS (for apoptosis-related protein in the TGF-beta signalling pathway). ARTS is expressed in many cells and acts to enhance cell death induced by TGF-beta or, to a lesser extent, by other apoptotic agents. Unlike related septin gene products, ARTS is localized to mitochondria and translocates to the nucleus when apoptosis occurs. Mutation of the P-loop of ARTS abrogates its competence to activate caspase 3 and to induce apoptosis. Taken together, these observations expand the functional attributes of septins previously described as having roles in cytokinesis and cellular morphogenesis.
Publication types
-
Research Support, Non-U.S. Gov't
-
Research Support, U.S. Gov't, P.H.S.
MeSH terms
-
Amino Acid Motifs
-
Amino Acid Sequence
-
Animals
-
Apoptosis / physiology*
-
Base Sequence
-
COS Cells
-
Caspases / metabolism
-
Cloning, Molecular
-
Cytoskeletal Proteins / chemistry
-
Cytoskeletal Proteins / genetics
-
Cytoskeletal Proteins / physiology*
-
DNA Primers / genetics
-
Enzyme Activation
-
GTP Phosphohydrolases / chemistry
-
GTP Phosphohydrolases / genetics
-
GTP Phosphohydrolases / physiology*
-
Gene Expression
-
Humans
-
Mitochondria / metabolism
-
Molecular Sequence Data
-
Septins
-
Sequence Homology, Amino Acid
-
Signal Transduction
-
Transfection
-
Transforming Growth Factor beta / physiology
Substances
-
Cytoskeletal Proteins
-
DNA Primers
-
Transforming Growth Factor beta
-
Caspases
-
GTP Phosphohydrolases
-
SEPTIN4 protein, human
-
Septins