cDNA micro array identification of a gene differentially expressed in adenovirus type 5- versus type 12-transformed cells

A C Vertegaal; H B Kuiperij; T van Laar; V Scharnhorst; A J van der Eb; A Zantema

doi:10.1016/s0014-5793(00)02339-5

cDNA micro array identification of a gene differentially expressed in adenovirus type 5- versus type 12-transformed cells

FEBS Lett. 2000 Dec 29;487(2):151-5. doi: 10.1016/s0014-5793(00)02339-5.

Authors

A C Vertegaal¹, H B Kuiperij, T van Laar, V Scharnhorst, A J van der Eb, A Zantema

Affiliation

¹ Laboratory for Molecular Carcinogenesis, MGC-Department of Molecular Cell Biology, Leiden University Medical Center, Wassenaarseweg 72, 2333 AL, Leiden, The Netherlands.

PMID: 11150499
DOI: 10.1016/s0014-5793(00)02339-5

Abstract

Proteins encoded by non-oncogenic adenovirus type 5 and oncogenic adenovirus type 12 differentially affect expression of a number of cellular genes. We have used cDNA micro array analysis to identify a cellular gene that is expressed in Ad12- but not in Ad5-transformed cells. This cellular gene was found to be the gene encoding follistatin-related protein, a TGF-beta inducible gene. Consistently, a constitutive factor binding to Smad binding elements was found in adenovirus type 12-transformed cells.

Publication types

Comparative Study
Research Support, Non-U.S. Gov't

MeSH terms

Adenoviruses, Human / genetics*
Cell Line, Transformed
Cell Transformation, Viral*
Expressed Sequence Tags
Follistatin-Related Proteins
Gene Expression Regulation* / drug effects
Glycoproteins / genetics*
Humans
Kidney
Lung
Oligonucleotide Array Sequence Analysis
Retina
Species Specificity
Transforming Growth Factor beta / pharmacology

Substances

Follistatin-Related Proteins
Glycoproteins
Transforming Growth Factor beta