Abstract
A Klebsiella pneumoniae strain resistant to cefoxitin and oxyimino-cephalosporins was cultured from a child hospitalized in Paris, France, in 1992. This isolate harboured a beta-lactamase gene located on an approximately 200 kb non-self-transferable plasmid. The beta-lactamase identified, DHA-2, shared 99% amino acid identity with the AmpC enzyme of Morganella morganii. DHA-2 was a point-mutant derivative of DHA-1 identified previously in a Salmonella enteritidis isolate. DHA-2 expression was inducible due to an ampR regulatory gene. This is the first report of an inducible and plasmid-located cephalosporinase from K. pneumoniae.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Bacterial Proteins / analysis
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Bacterial Proteins / metabolism
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Ceftazidime / pharmacology
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Cephalosporins / pharmacology
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Drug Resistance, Microbial
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Enzyme Induction / drug effects
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Isoelectric Focusing
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Klebsiella pneumoniae / enzymology*
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Klebsiella pneumoniae / genetics*
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Microbial Sensitivity Tests
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Molecular Sequence Data
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Phenotype
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Plasmids / genetics*
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Reverse Transcriptase Polymerase Chain Reaction
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beta-Lactamases / analysis
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beta-Lactamases / biosynthesis*
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beta-Lactamases / genetics*
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beta-Lactamases / metabolism
Substances
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Bacterial Proteins
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Cephalosporins
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Ceftazidime
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cephalosporinase DHA-2
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beta-Lactamases