Abstract
Incomplete sterilization of Mycobacterium tuberculosis Erdman cultures followed 1 h of incubation in low concentrations of glutaraldehyde (0.5 and 1.0%) or azide. In contrast, 2.5% glutaraldehyde, paraformaldehyde (2 or 4%), Vesphine IIse or 5% formalin sterilized these samples after 1 h. These results suggest caution in removing fixed M. tuberculosis samples from biosafety level 3.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Azides
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Bacteriological Techniques
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Disinfectants
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Fixatives
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Formaldehyde
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Glutaral
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Humans
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Laboratories / standards*
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Mycobacterium tuberculosis* / growth & development
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Polymers
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Safety
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Sterilization / methods*
Substances
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Azides
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Disinfectants
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Fixatives
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Polymers
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Formaldehyde
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Glutaral
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paraform