Abstract
The free ceramide content of rat liver mitochondria was found to be 1.7 nmol/mg protein and outer membranes contained a three-fold higher concentration than inner membranes. The mitochondrial content in neutral glycolipids was 0.6 nmol/mg protein. The long-chain bases found in free ceramides were d18:1 sphingosine, d18:0 3-ketosphinganine and t21:1 phytosphingosine in increasing order. In contrast, 3-ketosphinganine was the only base of glucosylceramide and lactosylceramide of inner membranes, whereas d18:1 sphingosine was the major long-chain base of glucosylceramide of outer membranes.
MeSH terms
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Animals
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Antigens, CD*
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Ceramides / analysis*
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Ceramides / chemistry*
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Fatty Acids / analysis
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Gas Chromatography-Mass Spectrometry
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Glucosylceramides / analysis
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Glucosylceramides / chemistry
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Intracellular Membranes / chemistry
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Lactosylceramides / analysis
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Lactosylceramides / chemistry
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Mitochondria, Liver / chemistry*
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Neutral Glycosphingolipids / analysis*
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Neutral Glycosphingolipids / chemistry*
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Rats
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Sphingosine / analogs & derivatives*
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Sphingosine / analysis
Substances
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Antigens, CD
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Ceramides
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Fatty Acids
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Glucosylceramides
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Lactosylceramides
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Neutral Glycosphingolipids
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ketodihydrosphingosine
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CDw17 antigen
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phytosphingosine
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Sphingosine