Insertion with long target duplication: a mechanism for gene mobility suggested from comparison of two related bacterial genomes

Gene. 2000 Dec 23;259(1-2):99-108. doi: 10.1016/s0378-1119(00)00456-x.

Abstract

The complete genome sequences of two closely related organisms--two Helicobacter pylori strains--have recently become available. Comparison of these genomes at single base pair level has suggested the presence of a mechanism for bacterial gene mobility--insertion with long target duplications. This mechanism is formally similar to classical transposon insertion, but the duplication is much longer, often in the range of 100bp. Restriction and/or modification enzyme genes are often within or adjacent to the insertion. A similar process may have mediated insertion of the cag(+) pathogenicity island in H. pylori. A similar structure was identified in comparisons between Neisseria meningitidis and Neisseria gonorrhoeae genomes. We hypothesize that this mechanism, as well as two other types of polymorphism linked with restriction-modification genes (insertion accompanied by target deletion and a tripartite structure composed of substitution/inversion/deletion), have resulted from attack by restriction enzymes on the chromosome.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Base Sequence
  • Chromosome Inversion
  • DNA Restriction-Modification Enzymes / genetics
  • DNA, Bacterial / genetics*
  • Genetic Variation
  • Genome, Bacterial
  • Helicobacter pylori / enzymology
  • Helicobacter pylori / genetics
  • Models, Genetic
  • Molecular Sequence Data
  • Mutagenesis, Insertional*
  • Neisseria meningitidis / enzymology
  • Neisseria meningitidis / genetics
  • Polymorphism, Genetic
  • Sequence Deletion

Substances

  • DNA Restriction-Modification Enzymes
  • DNA, Bacterial