Abstract
Kcc4, a kinase of the budding yeast Saccharomyces cerevisiae, is homologous to the bud neck protein kinases Hsl1/Nik1 and Gin4. We report here that a GFP-Kcc4 fusion protein is localized at the bud neck and that the non-kinase domain is required for this localization. We also demonstrate that Kcc4 associates with septin proteins in vitro and in vivo by two-hybrid analysis, GST pull-down experiments, immunoprecipitation, and analysis of direct association with affinity-purified GST-Kcc4 and MBP-Septin proteins. From the results obtained here, we suggest that Cdc11 is the primary association partner of Kcc4.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Cell Cycle Proteins / genetics
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Cell Cycle Proteins / metabolism*
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Cytoskeletal Proteins*
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Fungal Proteins / genetics
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Fungal Proteins / metabolism*
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Glutathione Transferase / genetics
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Glutathione Transferase / metabolism
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Green Fluorescent Proteins
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Immunoblotting
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Luminescent Proteins / genetics
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Luminescent Proteins / metabolism
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Microscopy, Fluorescence
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Protein Binding
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Protein Kinases / genetics
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Protein Kinases / metabolism*
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Recombinant Fusion Proteins / genetics
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Recombinant Fusion Proteins / metabolism
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Saccharomyces cerevisiae / genetics
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Saccharomyces cerevisiae / metabolism*
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Saccharomyces cerevisiae Proteins*
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Two-Hybrid System Techniques
Substances
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CDC11 protein, S cerevisiae
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Cell Cycle Proteins
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Cytoskeletal Proteins
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Fungal Proteins
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Luminescent Proteins
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Recombinant Fusion Proteins
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Saccharomyces cerevisiae Proteins
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Green Fluorescent Proteins
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Glutathione Transferase
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Protein Kinases
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KCC4 protein, S cerevisiae