Convenient genotyping of six myostatin mutations causing double-muscling in cattle using a multiplex oligonucleotide ligation assay

L Karim; W Coppieters; L Grobet; A Valentini; M Georges

doi:10.1046/j.1365-2052.2000.00684.x

Convenient genotyping of six myostatin mutations causing double-muscling in cattle using a multiplex oligonucleotide ligation assay

Anim Genet. 2000 Dec;31(6):396-9. doi: 10.1046/j.1365-2052.2000.00684.x.

Authors

L Karim¹, W Coppieters, L Grobet, A Valentini, M Georges

Affiliation

¹ Department of Genetics, Faculty of Veterinary Medicine, University of Liège, Belgium.

PMID: 11167526
DOI: 10.1046/j.1365-2052.2000.00684.x

Abstract

We herein describe a procedure that allows for simultaneous genotyping of six loss-of-function mutations in the bovine myostatin gene associated with the double-muscling phenotype. The proposed method relies on a multiplex oligonucleotide ligation assay and detection of the fluorescently labelled products using automatic sequencers.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Amino Acid Sequence
Animals
Base Sequence
Cattle / genetics*
DNA Primers
Genetic Techniques*
Muscle Development*
Muscles / metabolism
Mutation*
Myostatin
Oligonucleotides
Transforming Growth Factor beta / genetics*

Substances

DNA Primers
Myostatin
Oligonucleotides
Transforming Growth Factor beta