Adr1p-dependent regulation of the oleic acid-inducible yeast gene SPS19 encoding the peroxisomal beta-oxidation auxiliary enzyme 2,4-dienoyl-CoA reductase

Mol Cell Biol Res Commun. 2000 Aug;4(2):81-9. doi: 10.1006/mcbr.2000.0261.

Abstract

The role of Saccharomyces cerevisiae Adr1p was examined with respect to the transcriptional regulation of the SPS19 gene encoding the peroxisomal beta-oxidation auxiliary enzyme 2,4-dienoyl-CoA reductase. The SPS19 promoter contains both an oleate response element that binds the Pip2p-Oaf1p transcription factor as well as a canonical Adr1p-binding element, termed UAS1(SPS19). Northern analysis demonstrated that transcriptional up-regulation of SPS19 was abolished in cells devoid of Adr1p. Expression of an SPS19-lacZ reporter gene was shown to be quiescent in the adr1Delta mutant and abnormally elevated in cells containing multiple ADR1 copies. UAS1(SPS19) was able to compete for formation of a specific complex between recombinant Adr1p-LacZ and UAS1(CTA1) representing the corresponding Adr1p-binding element in the promoter of the catalase A gene, and to interact directly with this fusion protein. We conclude that in the presence of fatty acids in the medium transcription of SPS19 is directly regulated by both Pip2p-Oaf1p and Adr1p.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Binding Sites
  • Blotting, Northern
  • DNA Primers / chemistry
  • DNA-Binding Proteins / pharmacology*
  • Electrophoresis, Agar Gel
  • Fatty Acid Desaturases / genetics
  • Fatty Acid Desaturases / metabolism*
  • Fungal Proteins / metabolism*
  • Gene Expression Regulation, Fungal / drug effects
  • Genetic Vectors
  • Lac Operon / physiology
  • Oleic Acid / metabolism*
  • Oxidoreductases Acting on CH-CH Group Donors*
  • Peroxisomes / enzymology*
  • Peroxisomes / genetics
  • Recombinant Fusion Proteins / genetics
  • Recombinant Fusion Proteins / metabolism
  • Saccharomyces cerevisiae / drug effects
  • Saccharomyces cerevisiae / physiology*
  • Saccharomyces cerevisiae Proteins*
  • Spores, Fungal
  • Transcription Factors / genetics
  • Transcription Factors / metabolism*
  • Transcription Factors / pharmacology*
  • Transcriptional Activation
  • Up-Regulation
  • beta-Galactosidase / metabolism

Substances

  • ADR1 protein, S cerevisiae
  • DNA Primers
  • DNA-Binding Proteins
  • Fungal Proteins
  • Recombinant Fusion Proteins
  • Saccharomyces cerevisiae Proteins
  • Transcription Factors
  • Oleic Acid
  • Fatty Acid Desaturases
  • Oxidoreductases Acting on CH-CH Group Donors
  • 2,4-dienoyl-CoA reductase
  • beta-Galactosidase