Abstract
The K8 protein of Kaposi's sarcoma-associated herpesvirus (KSHV)/human herpesvirus-8 is a member of the bZIP family of transcription factors, which has homology with the Epstein-Barr virus transcription and replication factor, EB1. In this report, we have analysed the subcellular localization of the K8 protein and characterized a 12 amino acid sequence rich in basic residues which is responsible for targeting the protein to the cell nucleus. Furthermore, we show that a K8 mutant lacking the nuclear localization sequence can be directed to the nucleus by co-expression with an intact K8 protein, suggesting that K8 homodimerizes in the cytoplasm of the cell in vivo.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acids
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Animals
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Basic-Leucine Zipper Transcription Factors
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Binding Sites
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Carrier Proteins / biosynthesis
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Carrier Proteins / genetics
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Carrier Proteins / metabolism*
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Cell Nucleus / metabolism
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Cell Nucleus / virology*
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HeLa Cells
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Herpesvirus 8, Human / genetics
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Herpesvirus 8, Human / metabolism*
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Humans
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Leucine Zippers / genetics
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Mutagenesis
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Nuclear Localization Signals / biosynthesis
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Nuclear Localization Signals / genetics
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Nuclear Localization Signals / metabolism*
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Peptide Fragments
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Rabbits
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Recombinant Fusion Proteins / biosynthesis
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Recombinant Fusion Proteins / genetics
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Recombinant Fusion Proteins / metabolism
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Repressor Proteins
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Sarcoma, Kaposi / virology
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Transcription Factors / biosynthesis
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Transcription Factors / genetics
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Transcription Factors / metabolism*
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Viral Proteins / biosynthesis
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Viral Proteins / genetics
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Viral Proteins / metabolism*
Substances
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Amino Acids
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Basic-Leucine Zipper Transcription Factors
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Carrier Proteins
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K8 protein, Human herpesvirus 8
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Nuclear Localization Signals
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Peptide Fragments
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Recombinant Fusion Proteins
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Repressor Proteins
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Transcription Factors
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Viral Proteins