Effects of denervation on calcium channels in slow skeletal muscle fibers in the frog (Rana pipiens) were studied using the three-microelectrode voltage-clamp technique in intact fibers. Ca2+, Ba2+, and Sr2+ currents were all significantly reduced in amplitude during the first 2 weeks after denervation. After nerve section the selectivity sequence Ba congruent with Ca > Sr was changed to Ba > Sr > Ca and the values for relative ratio increased from 1.04 to 2.65 for Ba2+ and from 0.58 to 1.20 for Sr2+ (with respect to Ca2+). Barium current saturation was more obvious in denervated fibers than in non-denervated fibers. The values obtained with the Michaelis-Menten type expression, I = Imax/(1+Kd/[Ba]e) were Kd = 2.7 mM and Imax = 20 microA/cm2 in fibers 2 weeks after nerve section compared with the values Kd = 4.4 mM and Imax = 60 microA/cm2 obtained in non-denervated fibers. Additionally, the effects of two calcium channel blockers (cobalt and nifedipine) were greater by a factor of two in denervated fibers than in non-denervated fibers. Three weeks or so after nerve section, all the biophysical properties studied began to show a tendency to recover toward the values obtained in non-denervated muscles (controls). These results suggest that calcium channels are modified or that there is a change in the types of calcium channels present in frog slow skeletal muscle fibers after denervation.