Abstract
We assessed the ability of gene transfer to reverse vancomycin resistance in class A (VanA) glycopeptide-resistant Enterococcus faecalis. Recombinant shuttle vectors containing a vanH promoter-vanA antisense gene cassette fully restored vancomycin susceptibility through a combined transcriptional activator binding domain decoy and inducible vanA antisense RNA effect.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Anti-Bacterial Agents / pharmacology*
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Bacterial Proteins / antagonists & inhibitors
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Bacterial Proteins / genetics
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Carbon-Oxygen Ligases / antagonists & inhibitors
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Carbon-Oxygen Ligases / genetics
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Drug Interactions
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Enterococcus faecalis / drug effects
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Enterococcus faecalis / genetics*
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Gene Transfer Techniques
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Genetic Vectors
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Microbial Sensitivity Tests
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Molecular Sequence Data
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Oligonucleotides, Antisense / genetics
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Oligonucleotides, Antisense / pharmacology*
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Transformation, Bacterial
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Vancomycin / pharmacology*
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Vancomycin Resistance / genetics*
Substances
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Anti-Bacterial Agents
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Bacterial Proteins
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Oligonucleotides, Antisense
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VanA ligase, Bacteria
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Vancomycin
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Carbon-Oxygen Ligases